Anti-GABAA Receptor α1 Antibody

32160702

NA.41

The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 6 different isoforms of the alpha subunit, and receptor subunit expression varies during development. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency.

This antibody recognizes the topographical domain of GABAA Receptor α1.
Other homologies: Human (94% sequence homology).

Epitope: Topological domain.
KLH-conjugated linear peptide corresponding to the topological domain of rat GABAA Receptor α1.

Research Category
Neuroscience
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABAA Receptor α1 in rat hippocampus tissue.

Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated GABAA Receptor α1 in rat brain microsomal preparation RIPA lysate.
Research Sub Category
Neurotransmitters & Receptors
This Anti-GABAA Receptor α1 Antibody is validated for use in IH, IP, WB for the detection of GABAA Receptor α1.

Evaluated by Western Blot in rat brain microsomal preparation tissue lysate.

Western Blot Analysis: A 1:10,000 dilution of this antibody detected GABAA Receptor α1 in 10 µg of rat brain microsomal preparation tissue lysate.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
Rat Brain Microsomal Preparation Tissue Lysate.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

rat, mouse

Upstate^®

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Np_899155

P62813

wet ice

~51 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1