Anti-PKCε Antibody

32160702

NA.41

Members of the protein kinase C family fall into three categories: conventional (PKC alpha, beta I, beta II, gamma), novel (delta, epsilon, eta, mu, theta), and atypical (lambda, zeta). Conventional PKC isoforms are dependent on calcium and diacylglycerol (DAG) for their activity, whereas novel PKC isoforms are calcium dependent, and atypical PKC isoforms are calcium and DAG independent.

Recognizes PKCε, Mr 95 kDa.
Predicted to cross-react with rabbit.

KLH-conjugated, synthetic peptide corresponding to the C-terminal variable (V5) region, amino acids 726-737 (KGFSYFGEDLMP), of mouse Protein Kinase C Epsilon (PKCε). The immunizing sequence is identical in human, mouse, rat and rabbit.

Research Sub Category
Cytoskeletal Signaling
Research Category
Signaling
Immunoprecipitation:
An independent lab has reported this antibody immunoprecipitates PKCε.

Immunohistochemistry:
An independent lab has reported this antibody detects PKCε.
Detect PKCε using this Anti-PKCε Antibody validated for use in IH, IP & WB.

Routinely evaluated by Western Blot on Jurkat cell lysate.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected PKCε in Jurkat cell lysate. 0.5-2 μg/mL of a previous lot detected PKCε in a RIPA lysate from Jurkat cells, mouse brain extract and rat brain extract.

Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.
Store at -20°C.

Control
Jurkat cell lysate. Included Positive Antigen Control: Catalog # 12-303, Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

purified immunoglobulin

primary antibodies

polyclonal

mouse, human, rat

Upstate^®

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG

NM_005400

Q02156

dry ice

human ... PRKCE(5581)

Approx. 95

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1