Anti-FOXO3a/FKHRL1 Antibody

32160702

NA.41

The forkhead family of transcription factors is a large group of proteins that share a common conserved 100 amino acid DNA binding domain, which is termed a winged-helix or forkhead domain after the founding member of this group, the forkhead gene in Drosophila. In addition to their roles during normal development, recent studies have demonstrated that members of the forkhead family may participate in several kinds of neoplasia. In particular, 3 genes of the human forkhead family, FKHR, FKHRL1, and AFX, which are orthologues of DAF-16 in Caenorhabditis elegans, were identified at the chromosomal breakpoints in human neoplasms. These three genes have been assigned to the FOXO (named for “Forkhead Box, group O”) subfamily of forkhead transcription factors. The forkhead factors, including FKHRL1, regulate their activation through phosphorylation by proto-oncogene Akt (protein kinase B) and/or SGK (serum- and glucocorticoid- induced kinases) in mammalian cells and the phosphorylated form localized at the cytoplasm is recognized to be an inactivated form, whereas nuclear-imported dephosphorylated FKHRL1 is functional as an active transcriptional factor.

Not tested on other species.
Recognizes FKHRL1/FOXO3A, Mr 90-95 kDa.

Epitope: a.a. 330-673
GST fusion protein corresponding to residues 330-673 of human FKHRL1/FOXO3A.

Research Sub Category
Transcription Factors
Use Anti-FOXO3a/FKHRL1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect FOXO3a/FKHRL1 also known as AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, forkhead box O3A.
Research Category
Epigenetics & Nuclear Function

Routinely evaluated by Western Blot on 3T3/A31 cell lysates.

Western Blot Analysis:
A 0.5 g/mL dilution of this antibody was used to detect FOXO3a/FKHRL1 in 3T3/A31 cell lysate.

Replaces: 04-1007

Antigen affinity purified rabbit polyclonal, followed by anti-GST depletion, in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide.
Format: Purified
Affinity purified

Control
3T3/A31 Cell Lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

mouse, human

Upstate^®

western blot: suitable

IgG

NM_001455

O43524

wet ice

Approx. 90-95 kDa

Stable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

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12 - Non Combustible Liquids

WGK 1