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Anti-PDGF-BB Antibody

产品编号:4088483
规格:from rabbit, purified by affinity chromatography
包装规格:50 μg
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
408848350 μg5520
产品别名

Anti-PDGF-BB Antibody

Platelet-derived growth factor B chain precursor, Platelet-derived growth factor beta polypeptide, Platelet-derived growth factor, beta polypeptide (oncogene SIS), oncogene SIS, Platelet-derived growth factor, Platelet-derived growth factor beta, Platele

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Platelet-Derived Growth Factor (PDGF) is a dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB). Its receptors are transmembrane receptor tyrosine kinases, PDGF Receptors type A and B. The type A PDGF receptor binds all three PDGFs with high affinity, whereas the type B PDGF receptor is selective for PDGF-BB. PDGF signals as a growth and survival factor, as it is a potent activator of the PI3 Kinase pathway. PDGF signaling appears to play an essential role in the development of tumors of glial origin.
Specificity【特异性】
Reacts with human PDGF-BB.
Immunogen【免疫原】
Highly purified (>98%) recombinant human PDGF-BB
Application【应用】
Research Sub Category
Growth Factors & Receptors
Research Category
Signaling
ELISA: 0.5 μg/mL dilution of a previous lot will allow detection (in direct ELISA) of
0.2-0.4 ng/well when human PDGF-BB is used to coat the plate.

Neutralization: To yield ½ maximal inhibition (ND50) of the biological activity of hPDGF-BB (2.5
ng/mL), a concentration of 0.03-0.04 μg/mL of a previous lot of this antibody was required.

Immunohistochemistry(paraffin): Anti-IGF-1R, representative staining-pattern and morphology on ductal breast carcinoma. Tissue was pretreated with EDTA, pH 8.0 for Epitope retrieval. A previous lot of this antibody was diluted to 1:200 (RT incubation), using IHC-Select Detection with HRP-DAB.

Optimal working dilutions must be determined by the end user.
Anti-PDGF-BB Antibody detects level of PDGF-BB & has been published & validated for use in WB, ELISA, NEUT.
Quality【质量】
Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis:
1:500 dilution of this lot detected PDGF-BB on 10 μg of Huvec lysates.
Linkage【联系】
Replaces: AB1487P
Physical form【外形】
Purified rabbit polyclonal IgG lyophilized from 0.5X PBS, pH 7.4. Reconstitute with sterile distilled water.
Affinity purified
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
affinity purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
polyclonal
purified by【纯化方式】
affinity chromatography
species reactivity
human
technique(s)
ELISA: suitable
neutralization: suitable
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NM_002608
UniProt accession no.【UniProt登记号】
P01127
shipped in【运输】
dry ice
Gene Information
human ... PDGFB(5155)
产品说明
Target description【目标描述】
28 kDa
Storage and Stability【储存及稳定性】
Maintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 1
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