Anti-phospho Grb10 (Ser503) Antibody

32160702

NA.41

Grb10 (insulin receptor-binding protein Grb-IR) is a ubiquitous adapter protein which is expressed in both fetal and adult tissues. It is a multidomain protein that interacts with a wide array of cellular targets; the PH domain allows interaction with several subtypes of phosphoinositides; the Ras-associating domain binds a wide variety of Ras GTPases; and the SH domain facilitates binding to membrane-bound tyrosine kinase receptors which link Grb10 to different signaling pathways. Most notably, Grb10 associates with the insulin receptor at phosphotyrosine residues via its C-terminal BPS (between PH and SH) domain and inhibits the enzymatic activity of the insulin receptor. Grb10 also acts downstream of the insulin receptor pathway to inhibit activation of AKT-1. In addition, Grb10 suppresses Wnt signalling by binding to the low-density lipoprotein receptor. In fetal tissues, Grb10 may inhibit growth and placental size.

Other homologies: Rat (91% sequence homology).
This antibody recognizes Grb10 phosphorylated at (Ser503).

Epitope: Phosphorylated (Ser503)
KLH-conjugated linear peptide corresponding to human GRB10 phosphorylated at (Ser503).

This Anti-phospho Grb10 (Ser503) Antibody is validated for use in Peptide Inhibition Assay, Western Blotting for the detection of phospho Grb10 (Ser503).
Research Category
Signaling
Peptide Inhibition Assay Analysis: 1 µg/mL from a representative lot detected phospho-Grb10 (Ser503) on 10 µg of embryonic fibroblast cell lysate + non-phospho-Grb10 (Ser503) peptide. However, the antibody was inhibited when incubated with a phospho-Grb10 (Ser503) peptide.
Research Sub Category
Developmental Signaling

Evaluated by Western Blot in mouse embryonic fibroblast cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-Grb10 (Ser503) on 10 µg of mouse embryonic fibroblast cell lysate.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
Mouse embryonic fibroblast cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

mouse

porcine (based on 100% sequence homology), primate (based on 100% sequence homology), human (immunogen homology)

inhibition assay: suitable (peptide)
western blot: suitable

NP_005302

Q13322

wet ice

~75 kDa observed. An uncharacterized band appears at ~16 kDa in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1