Anti-trimethyl-Histone H4 (Lys20) Antibody

32160702

NA.41

Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.

This antibody recognizes the N-terminus of Histone H4 trimethylated at Lys20.

Epitope: Trimethylated Lys20
KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 trimethylated at Lys20.

Western Blot Analysis: 2 µg/mL from a representative lot detected trimethyl-Histone H4 (Lys20) in 10 µg of BALB3T3/A31 acid extract and RAT2 acid extract.

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see tables) were probed with Anti-trimethyl-Histone H4 (Lys20) (4 µg/mL).

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H4 using chromatin from HeLa cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated trimethyl-Histone H4 (Lys20) from HeLa cell lysate.
Anti-trimethyl-Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of trimethyl-Histone H4 (Lys20) also known as H4K20me3, Histone H4 (tri methyl K20) & has been validated in IP, WB, ChIP, DB.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.

Western Blot Analysis: 2 µg/mL of this antibody detected trimethyl-Histone H4 (Lys20) in 10 µg of HeLa acid extract.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
HeLa acid extract and various recombinant Histone proteins.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, mouse, rat

ChIP: suitable
dot blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NP_001029249

P62805

wet ice

~13 kDa observed. An uncharacterized band may appear at ~110 kDa in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1