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基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | IKK beta (IκB Kinase, IKKβ, I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. IκB Kinase (IKK) complex is a critical component in NFκB regulation. In a majority of unstimulated cells, the NFκB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IκB proteins. For NFκB to become activated, it must first disassociate from the inhibitor IκB. This occurs via the serine phosphorylation of IκB on Ser32 and Ser36. Post receptor stimulation, most notably by TNFα, NIK, a serine/threonine kinase, activates and phosphorylates IKKα and IKKβ. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates, which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1. IKK then binds to and phosphorylates IκBα on the two serine residues. This phosphorylation marks IκB for ubiquination and destruction by the proteosome. This frees the NFκB complex and allows it to translocate to the nucleus. |
Specificity【特异性】 | Recognizes IκBα, C-terminus. May react non-specifically with other proteins. Control peptide provided will compete only with the specific reaction of antiserum with IκBα. |
Immunogen【免疫原】 | Epitope: C-terminus IκBα peptide corresponding to a region near the C-terminus of the human protein, conjugated to KLH. |
Application【应用】 | Immunohistochemisty (paraffin): Representative testing from a previous lot. Anti-IкBα staining on invasive ductal carcinoma tissue (Breast Cancer) was pretreated with citrate buffer, pH 6.0. A 1:1,000 diluted was used using IHC-Select ELISA: Recommended Immunoprecipitation: Recommended Optimal dilutions must be determined by the end user. Research Sub Category Transcription Factors Detect IκBα using this Anti-IκBα Antibody validated for use in WB, IH(P), ELISA & IP. Research Category Epigenetics & Nuclear Function |
Quality【质量】 | Routinely evaluated by Western Blot on HEK293 lysates. Western Blot Analysis: 1:500 to 1:2,000 dilution of this lot detected IkBalpha on 10 μg of HEK293 lysates. |
Linkage【联系】 | Replaces: AB3016 |
Physical form【外形】 | Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium phosphate with 0.15 M NaCl, pH 7.2 and 0.1% sodium azide. |
Analysis Note【分析说明】 | Control HEK293 cell lysate. Control Peptide: Included with the antibody is 50 μg (1 mg/mL) of IκBα control peptide. The peptide will block the specific interaction of AB3016 with the IκBα subunit. Control peptide should be used at 1.0 μg per 1.0 μL of antiserum used in assay. Optimal concentrations must be determined by the end user. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | human, mouse, rat |
technique(s) | ELISA: suitable immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG |
NCBI accession no.【NCBI登记号】 | NM_020529.1 |
UniProt accession no.【UniProt登记号】 | P25963 |
shipped in【运输】 | dry ice |
Gene Information | human ... NFKBIA(4792) |
产品说明
Target description【目标描述】 | ~36 kDa |
Storage and Stability【储存及稳定性】 | Both antibody and control are stable for 6 months at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |