Anti-monomethyl-Histone H3 (Lys27) Antibody

32160702

NA.41

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.
Broad species cross-reactivity expected, based on sequence homology.

Epitope: Lys27
KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.

Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Anti-monomethyl-Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody to detect Histone H3 monomethylated at lysine 27. This purified Ab, also known as Anti-H3K27me1 is specificity verified by dot blot (DB), published in peer reviewed journals and validated in ICC, WB, ChIP, ChIP-seq.

Routinely evaluated by western blot acid in extracted proteins from Hela cells.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein A purified

Control
HeLa acid extract

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

purified antibody

primary antibodies

polyclonal

human, vertebrates

Upstate^®

ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable

IgG

NP_003484

Q16695

wet ice

human ... HIST1H3F(8968)

17 kDa

Stable for 1 year at 2-8°C from date of receipt.

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WGK 1