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Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| Specificity【特异性】 | Recognizes phospho-BMK1/Erk5 (Thr218, Tyr220). |
| Immunogen【免疫原】 | KLH-conjugated, synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5 |
| Application【应用】 | Research Sub Category MAP Kinases This Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody is validated for use in WB for the detection of phospho-BMK1/Erk5 (Thr218/Tyr220). Research Category Signaling Peptide Inhibition Analysis: HeLa cell lysate, HeLa cell lysate+ non-phosphorylated protein, and HeLa cell lysate + phosphorylated peptide were probed with Cat. No. 07-507, Anti-phospho-BMK 1/ERK5 (Thr218/Tyr220) (1µg/mL(1:1000) dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminesence detection system. Arrow indicates BMK1/ERK5 (~115 kDa) on HeLa cell lysate & HeLa cell lysate + non-phosphorylated peptide. No band is present on the HeLa cell lysate + phosphorylated peptide indicating successful peptide inhibition of the antibody. |
| Quality【质量】 | Evaluated by Western Blotting/Peptide Inhibition Analysis in HeLa cell lysate. Western Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected phospho-BMK1/Erk5 (Thr218/Tyr220) in HeLa cell lysate. |
| Physical form【外形】 | ImmunoAffinity Purified Affinity purified immunoglobulin in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide. |
| Analysis Note【分析说明】 | Control Sorbitol-treated HeLa cells |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | bovine, human, rat, mouse |
| manufacturer/tradename | Upstate® |
| technique(s) | inhibition assay: suitable (peptide) western blot: suitable |
| isotype【同位素/亚型】 | IgG |
| NCBI accession no.【NCBI登记号】 | NM_139033 |
| UniProt accession no.【UniProt登记号】 | Q13164 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | 110-115kDa |
| Storage and Stability【储存及稳定性】 | Maintain for 1 year at 2-8°C from date of shipment. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
