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产品别名
Anti-TAF3 Antibody
Transcription initiation factor TFIID subunit 3, 140 kDa TATA box-binding protein-associated factor, TBP-associated factor 3
Transcription initiation factor TFIID 140 kDa subunit, TAF(II)140, TAF140, TAF140, TAFII140
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | TAF3 is a subunit of TFIID that was initially identified through its interaction with a developmentally regulated transcription factor Bric a brac 1 (bab1), which is implicated in pattern formation and tissue morphogenesis in Drosophila. TFIID is a general or basal transcription factor that regulates initiation by allowing RNA polymerase II to recognize a core promotor. TAF3-TBPL2 complexes are found in myoblasts; however, upon differentiation into myotubes, they are replaced by TRF3-TAF3 complexes. Further research is still required to elucidate the roles and the development of the former to the latter complex. |
| Immunogen【免疫原】 | GST-tagged recombinant protein corresponding to human TAF3. |
| Application【应用】 | Research Sub Category Transcription Factors Research Category Epigenetics & Nuclear Function Detect TAF3 using this Anti-TAF3 Antibody validated for use in Chromatin Immunoprecipitation (ChIP) and Immunoprecipitation. Chromatin Immunoprecipitation: A representative lot immunoprecipitated TAF3 using chromatin from transfected cells grown under conditions that favor expression from the MyoD:EGFP transgene promoter over expression from the endogenous MyoD promoter. |
| Quality【质量】 | Evaluated by Immunoprecipitation in C2C12 cell lysate. Immunoprecipitation Analysis: A 1:1,000 dilution of this antibody detected TAF3 in 500 µg of C2C12 cell lysate. |
| Physical form【外形】 | Unpurified Rabbit polyclonal serum containing with 0.05% sodium azide. |
| Analysis Note【分析说明】 | Control C2C12 cell lysate. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | serum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | mouse |
| species reactivity (predicted by homology) | human, guinea pig, rhesus macaque |
| technique(s) | ChIP: suitable immunoprecipitation (IP): suitable |
| NCBI accession no.【NCBI登记号】 | NP_114129.1 |
| UniProt accession no.【UniProt登记号】 | Q5VWG9 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~180 kDa observed. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
