您的位置：首页 > 产品中心 > FnCas9 Protein
FnCas9 Protein
| 产品编号： | 4090167 |
| 规格： | from Francisella novicida, recombinant, expressed in E. coli, 1X NLS |
| 包装规格： | 50 μG，250 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
FnCas9 Protein
Francisella Novicida Cas9
产品性质
| Quality Level【质量水平】 | 200 |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥95% (SDS-PAGE) |
| form【形式】 | lyophilized powder |
| application(s) | CRISPR |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | pkg of 50 μg (≥ 300 pmol) pkg of 250 μg (≥ 1500 pmol) |
基本信息
| General description【一般描述】 | The purified FnCas9 protein (~190 kDa) is derived from the type II CRISPR/Cas system from the bacterium Francisella novicida and has been engineered to function using three molecular components: the FnCas9 protein, a crRNA of user-defined sequence, and a tracrRNA of constant sequence. The FnCas9 endonuclease can be programmed by changing the crRNA sequence to create a site-specific double-strand break (DSB) in plasmids or purified genomic DNA. |
| Application【应用】 | Functional Genomics/Target Validation/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology |
| Features and Benefits【特点和优势】 |
|
| Principle【原理】 | Conventional restriction enzymes such as EcoRI and HindIII can only cut specific short (6 bp) DNA sequences and are therefore limited to cloning small DNA fragments (typically ~4 kb). CRISPR-based endonculeases can cut user defined sequences of a longer length (~20 bp) making them more flexible and applicable to larger DNA fragments such as multigene bacterial operons or mammalian genomic DNA with native promoter, exon, and intron components. |
| Reconstitution【重悬】 | Lyophilized FnCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube. |
| Other Notes【其他说明】 | Click here to order gRNA Enter the 20bp genomic DNA target sequence (5′ to 3′) plus the adjacent 3′ PAM site. Under special instructions, indicate "FnCas9 crRNA" Click here to order FnCas9 tracrRNA Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins |
| Legal Information【法律信息】 | CRISPR Use License Agreement MISSION is a registered trademark of sigma-aldrich Co. LLC |
| Components【组分】 | 组份不可单独销售 FnCas9-NLS from Francisella novicida, expressed in Escherichia coli Dilution buffer for Cas9 proteins Reconstitution solution for Cas9 proteins Digestion buffer 10× for FnCas9 |
安全信息
| Pictograms【象形图】 | GHS07 |
| Signal word【警示用语：】 | Warning |
| Hazard Statements | H319 |
| Precautionary Statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| Hazard Classifications【危险分类】 | Eye Irrit. 2 |
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
