抗 KLF5(Krüppel 样因子 5)抗体

32160702

NA.41

Krüppel-like factors (KLFs) are zinc finger-containing transcription factors with regulatory functions in cell growth, proliferation, differentiation, and embryogenesis. KLF5, (also known as BTEB2 and IKLF), is essential for mouse embryo development. KLF5 activates many genes during cardiovascular remodeling, including PDGFa/b, Egr-1, PAI-1, iNOS, and VEGF receptors, making KLF a potential therapeutic target for cardiovascular disease. KLF5 also plays a role in angiogenesis and in development of several tumor types including breast, prostate, bladder, colon, esophagus, and skin. KLF5 is closely related to other of the KLF family members. However, KLF5 and KLF4 exhibit opposite effects on regulation of gene transcription, despite binding to similar, if not identical, cis-acting DNA sequences. In addition, KLF4 and 5 exert contrasting effects on cell proliferation in many instances; while KLF4 is an inhibitor of cell growth, KLF5 stimulates proliferation.

This antibody recognizes Krüppel-like factor 5 at the N-terminus.

Recombinant protein corresponding to mouse Krüppel-like factor 5 at the N-terminus.
Epitope: N-terminus

Research Sub Category
Transcription Factors

Pluripotent & Early Differentiation
Anti-KLF5 (Krüppel-like factor 5) Antibody is a Rabbit Polyclonal Antibody for detection of KLF5 (Krüppel-like factor 5) also known as Krüppel-like factor 5 & has been validated in WB.
Chromatin Immunoprecipitation Analysis (ChIP):
A representative lot was used by an independent laboratory in ChIP. (Ng Huck Hui, Genome Institute of Singapore)
Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Evaluated by Western Blot in mouse embryonic stem cell chromatin extract.

Western Blot Analysis: 1:2,000 dilution of this antibody detected Krüppel-like factor 5 on 10 µg of mouse embryonic stem cell chromatin extract.

Unpurified
Rabbit polyclonal serum containing 0.05% sodium azide.

Control
Mouse embryonic stem cell chromatin extract

rabbit

100

serum

primary antibodies

polyclonal

mouse

ChIP: suitable
western blot: suitable

NP_033899

Q9Z0Z7

wet ice

human ... KLF5(688)

~55 kDa observed. An uncharacterized band appears at ~30 kDa

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1