Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate

32160702

NA.41

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Recognizes Histone H2A.X phosphorylated at Ser139, MW 15 kDa.
Broad species cross-reactivity is expected based on conservation of sequence homology.

KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse. Clone JBW301.
Epitope: Ser139

Research Sub Category
Histones
Use Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate (mouse monoclonal antibody) validated in FC, ICC to detect phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry: 2-4 μg/mL of a previous lot detected phospho-histone H2A.X in etoposide-treated HeLa cells fixed with 95% ethanol/5% acetic acid (10 minutes), followed by 1% formaldehyde, 0.25% Triton^™ X-100 in TBS (5 min.).

Evaluated by Flow Cytometry to detect Log phase Jurkat cells treated with staurosporine.

Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.

Purified FITC-conjugated mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide. Frozen solution.
Protein G Purified

Control
Staurosporine-treated Jurkat cells

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

FITC conjugate

purified immunoglobulin

primary antibodies

JBW301, monoclonal

human

Upstate^®

flow cytometry: suitable
immunocytochemistry: suitable

IgG1

NM_002105.2

P16104

dry ice

15 kDa

Stable for 1 year at from date of receipt.

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WGK 2