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TNF-α human
| 产品编号： | 4090707 |
| 规格： | Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture |
| 包装规格： | 50 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
TNF-α human
Tumor necrosis factor
Cytotoxin
Differentiation-inducing factor (DIF)
Necrosin
Cachectin
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | TNF-α (tumor necrosis factor α) is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. The gene is mapped to human chromosome 6p21.3. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. |
| Application【应用】 | TNF-α (tumor necrosis factor α) human has been used as a positive control for induction of proinflammatory genes in human intestinal and hepatic cell lines. |
| Biochem/physiol Actions【生化/生理作用】 | TNF-α (tumor necrosis factor α) is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. However, it can be a tumor promoter as well as a tumor suppressor. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, apoptosis and diabetes. It is also associated with mood and anxiety disorders. |
| Sequence【序列】 | VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL |
| Physical form【外形】 | Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 0.5mM DTT. |
| Reconstitution【重悬】 | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20℃ to -80℃. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥98% (HPLC) ≥98% (SDS-PAGE) |
| form【形式】 | lyophilized |
| potency【效能】 | ≤0.05 ng/mL ED50 |
| mol wt【分子量】 | 17.4 kDa |
| packaging【包装】 | pkg of 50 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities【杂质】 | <0.1 EU/μg endotoxin, tested |
| color【颜色】 | white |
| UniProt accession no.【UniProt登记号】 | P01375 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... TNF(7124) |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
