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Anti-Synapsin I Rabbit pAb
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | lyophilized |
does not contain【不包含】 | preservative |
species reactivity | human, mouse, rat, bovine |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze |
isotype【同位素/亚型】 | IgG |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 10 μg in Plastic ampoule |
基本信息
General description【一般描述】 | Recognizes the synapsin I protein in rat brain. Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein. This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I. |
Immunogen【免疫原】 | Bovine purified, brovine brain synapsin I |
Application【应用】 | ELISA (1:2500-1:10,000) Frozen Sections (see application references) Immunoblotting (1:200) Immunocytochemistry (1:500-1:2500) Immunoprecipitation (1 µg/200 µg lysate) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | Lyophilized from 5 mM ammonium bicarbonate. |
Reconstitution【重悬】 | Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. |
Analysis Note【分析说明】 | Positive Control Rat brain |
Other Notes【其他说明】 | This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution. Stone, L.M., et al. 1994. J. Neurosci.14, 301. Mandell, J.W., et al. 1992. J. Neurosci.12, 1736. Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617. Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |