Anti-Laminin alpha5 Antibody, clone 4C7 (Ascites Free)

32160702

NA.41

Laminin subunit alpha-5 (UniProt: O15230; also known as Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha) is encoded by the LAMA5 (also known as KIAA0533, KIAA1907) gene (Gene ID: 3911) in human. Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Thus far five alpha, three beta, and three gamma subunits are known, which assemble into at least 15 different laminin molecules. The alpha, beta, and gamma chains are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-5 is a subunit of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). It contains 22 laminin EGF-like domains, 5 5 laminin G-like domains, 1 laminin IV type A domain, and 1 laminin N-terminal domain. Laminin 5-alpha is expressed in heart, lung, kidney, muscle, pancreas, retina, and placenta. Very little or no expression is observed in brain and liver.

Clone 4C7 reacts with the globular domain of the alpha5 chain of intact laminin and blocks the neurite stimulating activity of laminin.

Full length purified human laminin alpha 5.

Research Category
Cell Structure
Immunohistochemistry Analysis: A representative lot detected Laminin alpha 5 in immunostained basement membranes in O.T.C.-embedded fetal and adult human lung cryosections (Pierce, R.A., et al. (2000). Am. J. Respir. Cell Mol. Biol. 23(6):742-747).

Neutralizing Analysis: A representative lot detected Laminin alpha 5 in adhesion of sickle cell patients (Hb SS) red blood cells (RBCs) to human placental laminin-coated surface (Lee, S.P., et al. (1998). Blood. 92(8):2951-2958).

ELISA Analysis: A representative lot detected Laminin alpha 5 in recombinant human (rh) Laminin-10 ( 5 1 1) and Laminin-11 ( 5 2 1), but not Laminin-8 ( 4 1 1) (Wondimu, Z., et al. (2013). PLoS One. 8(1):e53648).

Immunofluorescence Analysis: A representative lot detected Laminin alpha 5 in immunoreactivity in human skin and kidney cryosections (Wondimu, Z., et al. (2013). PLoS One. 8(1):e53648).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Laminin alpha 5 in Laminin-10 ( 5 1 1) from A549 culture medium (Wondimu, Z., et al. (2013). PLoS One. 8(1):e53648).

Anti-Laminin alpha5, clone 4C7 (Ascites Free), Cat. No. MAB1924-C, is a mouse monoclonal antibody that detects Laminin alpha 5 and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and neutralization.

Evaluated by Immunoprecipitation in A431 cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Laminin alpha 5 in 500 µg of A431 cell lysate.

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

100

mouse

purified antibody

primary antibodies

4C7, monoclonal

human

antibody small pack of 25 μg

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable

IgG2aκ

NP_005551

O15230

ambient

~260 kDa observed; 399.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1