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Cholesterol Oxidase from microorganisms
产品价格查看更多规格...
| 产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
| 4091357 | 100 UNITS | 3030 | |||
| 4091357 | 500 UNITS | 10900 |
产品别名
9028-76-6
Cholesterol Oxidase from microorganisms
Cholesterol:oxygen oxidoreductase
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 1.1.3.6 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 232-842-1 |
| MDL number | MFCD00130783 |
| NACRES | NA.54 |
| Application【应用】 | Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that determined that cholesterol depletion impairs coupling between channel opening and vesicle release by allowing voltage-gated calcium channels to move further from release sites. Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that evaluated the effects of cholesterol depletion. This study reported that cholesterol depletion impairs the coupling between channel opening and vesicle release. Additionally, cholesterol oxidase is used to determine serum cholesterol levels. The enzyme also finds application in the microanalysis of steroids in food samples. It has also been used for distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase have been investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures. |
| Biochem/physiol Actions【生化/生理作用】 | Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. The pH optimum of the enzyme is 7.0 to 7.5 and temperature optimum is 50 ℃. The pH stability is 5.7-7.8. Hg2+, Ag+, and ionic detergents inhibit the enzyme activity. Molecular mass of the enzyme is 62 kDa. Pathogenic bacteria require CHOD to infect a host′s macrophage. |
| Unit Definition【单位定义】 | One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 ℃. Note: 4-cholesten-3-one may undergo isomerization. |
| Preparation Note【制备说明】 | CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0. Prepare solutions immediately before use. |
产品性质
| Quality Level【质量水平】 | 200 |
| form【形式】 | aqueous solution |
| Specific Activity | ≥30 units/mg protein (biuret) |
| mol wt【分子量】 | 62 kDa |
| solubility【溶解性】 | 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold) |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
