FLT3 (571-993), active, GST tagged human

NA.32

FLT3 (fms-related tyrosine kinase 3) is a tyrosine kinase receptor and the gene is localized to human chromosome 13q12. The encoded protein is composed of 993 amino acids, and consists of five immunoglobulin (Ig)-like domains in its exoplasmic region. It is also composed of a transmembrane region, a juxtamembrane domain, and two tyrosine kinase domains in its intracellular domain. The tyrosine kinase domains are linked via a kinase-insert domain.

FLT3 (fms-related tyrosine kinase 3), upon interaction with its ligand, undergoes conformational changes to form homodimers. This results in phosphorylation and activation of downstream signaling pathways including phosphatidylinositol 3-kinase (PI3K/AKT), mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) and signal transducer and activator of transcription 5 (STAT5) pathways. Internal tandem duplication (ITD) mutation or a point mutation in juxtamembrane domain-coding region or within the activation loop domain, respectively, of this gene lead to its constitutive action. FLT3/ITD mutation is associated with later stages of acute myeloid leukemia (AML) pathogenesis. These patients have poor prognosis.

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

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200

expressed in baculovirus infected Sf9 cells

PRECISIO^® Kinase

≥70% (SDS-PAGE)

buffered aqueous glycerol solution

60-81 nmol/min·mg

~73 kDa

P36888

dry ice

−70℃

human ... FLT3(2322)

GHS07

Warning

H317 - H319 - H412

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Aquatic Chronic 3 - Eye Irrit. 2 - Skin Sens. 1

10 - Combustible liquids

WGK 3