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Milli-Mark® Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate

产品编号:4092593
规格:clone TRA-2-49/6E, Milli-Mark®, from mouse
包装规格:100 TESTS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4092593100 TESTS4680
基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
Specificity【特异性】
Antibody recognizes TRA-2-49.
Immunogen【免疫原】
2102Ep human Embryonal Carcinoma Cells
Epitope: Unknown
Application【应用】
Working Solution Cells / test Total Reaction Volume
5 μl 5 x 105 Cells / 95 μl PBS 100 μl
10 μl 1 x 106 Cells / 90 μl PBS 100 μl

Research Sub Category
Pluripotent & Early Differentiation
Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.
Research Category
Stem Cell Research
Quality【质量】
Evaluated by flow cytometry using 2102 Ep cells.
Physical form【外形】
Protein A purified
Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Analysis Note【分析说明】
Control
2102 Ep cells.
Legal Information【法律信息】
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
conjugate【偶联物】
FITC conjugate
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
TRA-2-49/6E, monoclonal
species reactivity
human
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
isotype【同位素/亚型】
IgG1
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
212/80/55 kDa Observed
Storage and Stability【储存及稳定性】
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
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