Milli-Mark^® Anti-TrkA-FITC Antibody

32160702

NA.41

TrkA is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.

Unconjugated version Cat.No.06-574 is predicted to react with Rat based on immunogen design. The unconjugated part number was also shown to react with Mouse.
Antibody recognizes TrkA protein.

Bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.
Epitope: Extracellular domain

Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases
Research Category
Neuroscience
Detect TrkA using this Milli-Mark Anti-TrkA-FITC Antibody validated for use in FC.

Evaluated by flow cytometry using HeLa cells.

Purified rabbit polyclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Protein A purified

Control
HeLa cells

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

FITC conjugate

purified immunoglobulin

primary antibodies

polyclonal

human

Milli-Mark^®

flow cytometry: suitable

IgG

NP_067600

P35739

wet ice

TrkA exists as two isoforms: 87 kDa and 88 kDa

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2