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Anti-HU-B27 Antibody, FITC conjugated

产品编号:4092609
规格:Chemicon®, from mouse
包装规格:1 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40926091 ML5350
产品别名

Anti-HU-B27 Antibody, FITC conjugated

Com-B27

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
HU-B27 (HLA- Com-B27) is involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.
Specificity【特异性】
This reagent binds strongly to the HLA-B27 antigen. It also binds weakly to the HLAB7 antigen. This formulation demonstrates less cross reactivity to HLA-B7 than Chemicon products, MAB1285 and MAB1285F.
Application【应用】
Detect HU-B27d using this Anti-HU-B27 Antibody, FITC conjugated validated for use in FC, IC, IF & WB.
Research Sub Category
Immunoglobulins & Immunology
Flow cytometry and Immunofluorescence: use 10 mL direct from the vial per 100 mL of whole blood, or 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 mL buffer.

Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects/Fluorescence Intensity
Heterozygous HLA-B27+/Strong
Homozygous HLA-B7+/Weak
Heterozygous HLA-B7+/Weak - negative
Non B27, Non B7/Negative

This reagent is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis.

SUGGESTED USAGE:
Flow cytometry and immunofluorescence - use 10 µl direct from the vial per 100 µl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 µl buffer.

Use Control IgG2a-FITC (MABC002F) as an isotype control.

Please note that this reagent should be used in a single-colour single-step assay only.
Research Category
Inflammation & Immunology
Quality【质量】
Evaluated by Western Blot on Jurkat lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected Com-B27 on 10 µg of Jurkat lysates.
Physical form【外形】
The reagent is supplied in 1.0 mL phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. Purified from mouse ascitic fluid. The characteristics of each lot are tested by gel electrophoresis and flow cytometry.
Analysis Note【分析说明】
Control
Control IgG2a-FITC (MABC002F) as an isotype control
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
conjugate【偶联物】
FITC conjugate
antibody form【抗体形式】
purified antibody
clone【克隆】
monoclonal
species reactivity
rat, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
isotype【同位素/亚型】
IgG2a
UniProt accession no.【UniProt登记号】
P03989
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
19 kDa
Storage and Stability【储存及稳定性】
Stable for 6 months at 2-8ºC in the dark from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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