MAPKAP Kinase 2 Protein, active, 10 μg

32160405

NA.41

Product Source: Human MAPKAP Kinase 2 expressed in E. coli.
Recombinant human MAPKAP Kinase 2, residues 46-400, A399G, containing an N-terminal GST tag.

MAPKAP Kinase 2 has been implicted as a cell cycle checkpoint kinase, joining the ranks of Chk1 and Chk2 as critical regulators of the DNA damage response in mammalian cells in recent studies (Manke, et al, 2005, and Abraham, 2005). This research indicates that MAPKAP Kinase 2, a serine/threonine kinase activated by p38, phosphorylates CDC25 B/C, generating a binding site for 14-3-3 proteins. The checkpoint kinases Chk1 and Chk2 are targets for drug discovery efforts designed to overcome cell cycle arrest due to DNA damage induced by chemotherapeutic agents. Overriding the checkpoint kinases in cancer cells exposed to DNA damaging agents leads to catastrophic failure of cell division and apoptosis.
The new role of MAPKAP Kinase 2 in complementing the function of Chk1 and Chk2 suggests a new target for drug discovery efforts.

Protein Target: MAPKAP-K2
Target Sub-Family: CAMK

Routinely evaluated by phosphorylation of MAPKAP Kinase 2 Substrate Peptide

GSH-agarose & Q-Sepharose FF

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

human

M_w 70.2 kDa

Upstate^®

activity assay: suitable (kinase)

NM_004759.3
NM_032960.2

P49137

human ... MAPKAPK2(9261)

Also available in 250μg size as catalog # 14-337M - Call for pricing and availability.

1 year at -20°C

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07

Warning

H317

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Skin Sens. 1

12 - Non Combustible Liquids

WGK 1