PI 3-Kinase (p110α(H1047R)/p85α), 10 μg

32160405

NA.32

The H1047R substitution is a somatic mutation in p110α that has been associated with tumours of the colon, stomach, breast and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation. (Samuels Y. et al., Science, (2004); 304: 554; Kang S. et al., PNAS, (2005);102: 802-807 and Zhao J.J. et al., PNAS, (2005);102:18443-18448).
Complex of N-terminal 6His-tagged recombinant human p110α full length, containing the mutation H1047R, and untagged, recombinant, human p85α full length.

Also available in 250μg size --call for pricing and availability and reference catalog number 14-792M when ordering the 250μg size.

50 mM Tris/HCl pH8.0, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Ni²⁺/NTA-agarose

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

100

human

M_w 125 kDa (p110α(H1047R))
M_w 84 kDa (p85α)

Upstate^®

P26450 (mouse)
P27986
P42336
P42337 (mouse)
U79143

human ... PIK3CA(5290)

Protein Target: PI3Kα/p85α
Target Sub-Family: PI3K/PI4K

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07

Warning

H317

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Skin Sens. 1

10 - Combustible liquids

WGK 1