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Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC: Pre-Screened for Angiogenesis &VEGF signaling, neonatal

产品编号:4095233
包装规格:1 EACH
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40952331 EACH12380
基本信息
NACRES
NA.81
General description【一般描述】
Endothelial cells (EC) line the blood vessels of the entire circulatory system, from aorta to capillaries, and act as a barrier between circulating blood and the rest of the vessel wall/surrounding tissues. Endothelial cells are involved in the processes of angiogenesis, vasculogenesis, vasoregulation, coagulation, fibrinolysis and selective blood barrier. They are also implicated in pathophysiological processes, including cardiovascular disease and cancer development.

The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). Select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx.

VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells
VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.

Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells
Prescreened Endothelial Cells express the following important biomarkers:
  • Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes; eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function;
  • Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation.
  • Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival.
Cell Line Origin【细胞系来源】
Blood Vessels
Application【应用】
Endothelial vessel function, cell signaling, angiogenesis
Components【组分】
Cell Basal Medium containing 10% FBS & 10% DMSO
Preparation Note【制备说明】
  • 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Subculture Routine【传代培养常规】
Please refer to the CADMEC/HMVEC Culture Protocol.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
human blood vessels
packaging【包装】
pkg of 500,000 cells
growth mode【生长模式】
Adherent
karyotype【核型】
2n = 46
morphology【形态学】
Endothelial
technique(s)
cell culture | mammalian: suitable
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 3
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