Goat Anti-Rat IgG Antibody, HRP conjugate, Species Adsorbed

32160702

NA.46

EIA and Western blots: 1:5,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000.

Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Goat anti-Rat IgG Antibody, HRP conjugate, Species Adsorbed detects level of Rat IgG & has been published & validated for use in ELISA & WB.
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Replaces: AP306P

Lyophilized. Buffer = 0.01 M sodium phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.

RECONSTITUTION:

Reconstitute with 500 μL of sterile distilled water.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

goat

peroxidase conjugate

F(ab′)₂ fragment of affinity isolated antibody

secondary antibodies

polyclonal

rat

Chemicon^®

0.8 mg/mL

ELISA: suitable
western blot: suitable

wet ice

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

13 - Non Combustible Solids

WGK 3