Anti-phospho-MYPT1 (Thr696) Antibody

32160702

NA.41

Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. It is also involved in smooth muscle contraction. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20 kDa Myosin light chain to MYPT1. PP1c interacts with at least four binding sites on the amino-terminus of MYPT1. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity.

This antibody recognizes phosphorylated MYPT1 at Thr696.

Epitope: Phosphorylated Thr696
KLH-conjugated linear peptide corresponding to human phosphorylated Thr696 of MYPT1.

Research Category
Signaling
Anti-phospho-MYPT1 (Thr696) Antibody is an antibody against phospho-MYPT1 (Thr696) for use in WB.
Western Blotting (SNAP ID)Analysis: 0.1 µg/mL from a previous lot detected MYPT1 on 10 µg of NIH/3T3 cell lysate.
Research Sub Category
Kinases & Phosphatases

Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody was used to detect MYPT1 phosphorylated at Thr696 on 10 µg of Calyculin/Okadaic treated and untreated HEK293 cell lysate.

Replaces: 07-251

Purified rabbit polyclonal containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
Affinity purified

Control
Calyculin/Okadaic treated and untreated HEK293 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, mouse, canine

dog, Xenopus, rat, primate, horse, chicken, Danio, bovine, monkey

western blot: suitable

NP_001137357.1

O14974

wet ice

Expected at 115 kDa and observed at 130 kDa

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1