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产品别名
Anti-dUTPase Antibody
Deoxyuridine 5′-triphosphate nucleotidohydrolase, mitochondrial, dUTPase, dUTP pyrophosphatase, dUTPase
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | dUTPase, also known as dUTP pyrophosphatase, and encoded by the gene DUT, is a critical enzyme involved in nucleotide metabolism for the production of dUMP, the immediate precursor of thymidine nucleotides. dUTPase normally can also function to decrease the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA and cause mutations. dUTPase is localized to the nucleus and mitochondrial locations. dUTPase is expressed in numerous tissues and has multiple isoforms. Some isoforms are constitutively expressed while others match DNA replication cycles. Because dUTPase plays a pivotal role in regulating cellular dUTP pools, this enzyme could have profound effects on the efficacy of agents that target thymidylate biosynthesis and dUTPase expression is being examined as a prognostic marker for overall survival and response to therapy in colon and other cancers where thymidylate therapy is used. |
| Immunogen【免疫原】 | Recombinant protein corresponding to human dUTPase. |
| Application【应用】 | Research Sub Category Apoptosis - Additional Anti-dUTPase Antibody is a highly specific rabbit polyclonal antibody, that targets dUTPase & has been tested in western blotting. Research Category Apoptosis & Cancer Western Blotting Analysis: 2 µg/mL from a representative lot detected dUTPase in 10 µg of A431, HEK293, and HeLa cell lysates and human placenta tissue lysate. Western Blotting Analysis: A representative lot from an independent laboratory detected dUTPase in MDA-MB231, MCF-7, H184B5F5/M10 cell lysate (Hu, C. M., et al. (2012). Cancer Cell. 22(1):36-50.). |
| Quality【质量】 | Evaluated by Western Blotting in MDA-MB-231 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected dUTPase in 10 µg of MDA-MB-231 cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein A purified |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_001020419 |
| UniProt accession no.【UniProt登记号】 | P33316 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... DUT(1854) |
产品说明
| Target description【目标描述】 | ~18 kDa observed. The expected molecular weight of this protein is 27 kDa However, this protein has been observed at 18 kDa (Prof. Z. F. Chang, National Yang-Ming University). Uncharacterized band(s) may be observed in some cell lysates. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
