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Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse
| 产品编号： | 4096464 |
| 规格： | SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture |
| 包装规格： | 10 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse
SDF-1α/PBSF
基本信息
| MDL number | MFCD02100631 |
| Quality【质量】 | endotoxin tested |
| General description【一般描述】 | Stromal derived factor-1 (SDF-1) is localized to adipocytes. |
| Application【应用】 | Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles. |
| Biochem/physiol Actions【生化/生理作用】 | Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis. SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α.. |
| Physical form【外形】 | Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin. |
| Preparation Note【制备说明】 | Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa. |
| Analysis Note【分析说明】 | The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4. |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | >97% (SDS-PAGE) |
| form【形式】 | powder |
| potency【效能】 | 0.15-0.6 ng/mL ED50 |
| mol wt【分子量】 | predicted mol wt ~8 kDa |
| packaging【包装】 | pkg of 10 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| impurities【杂质】 | <1 EU/μgtested (LAL test) |
| UniProt accession no.【UniProt登记号】 | P40224 |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | mouse ... Cxcl12(20315) |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
