BIOshell^TM A160 Peptide CN, 2.7 μm HPLC Column

SB.52

BIOshell^™ 160Å Peptide CN columns provide an alternative selectivity to the traditional C18 bonded phases. The introduction of the cyano group enables peptides and small proteins to be separated due to a combination of hydrophobicity and dipole-dipole interactions making this column an excellent choice for biomolecules that are not resolved on traditional C18 columns. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected cyanopropyl ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 ℃).

UHPLC Analysis of Peptides on BIOshell^™ A160 Peptide C18, CN, and Phenyl-Hexyl, 2.7 μm

BIOshell is a trademark of sigma-aldrich Co. LLC

100

stainless steel hardware (column)

suitable for USP L10

Shell thickness (0.5 μm)
Solid Core (1.7 μm)

BIOshell^™

endcapped

peptides

BIOshell^™

1 ea of

2.2% carbon loading

600 bar max. pressure
90 ℃ max. temp.

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

15 cm × 100 μm

2.2 μmol/m²

spherical silica particle platform
superficially porous particle

cyano phase

2.7 μm

160 Å

90 m²/g

1 - 8

hydrophilic interaction (HILIC)
normal phase
reversed phase