BIOshell^TM A400 Protein C18 Column

SB.52

BIOshell^™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core^® particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns offer maximum hydrophobicity for resolving tricky hydrophilic and PEGylated proteins. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C18 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 ℃, and within the pH range of 1 to 9.

UHPLC Analysis of Proteins on BIOshell^™ A160 Peptide C18 2.7 μm; BIOshell^™ A400 Protein C18, 3.4 μm; and BIOshell^™ IgG 1000 Å C18, 2.7 μm

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

BIOshell is a trademark of sigma-aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

100

stainless steel hardware (column)

suitable for USP L1

Shell Thickness (0.2 μm)
Solid Core (3.0 μm)

BIOshell^™

endcapped

proteins

BIOshell^™

1 ea of

1% carbon loading

600 bar max. pressure
90 ℃ max. temp.

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

15 cm × 4.6 mm

spherical silica particle platform
superficially porous particle

C18 (octadecyl) phase

3.4 μm

3.4 μm particle size
400 Å

~15 m²/g

1-8

reversed phase