Caspase-2, Human, Recombinant, E. coli

100

≥90% (SDS-PAGE)

lyophilized solid

≥1500 units/mg protein

Calbiochem^®

OK to freeze

PBS: 0.1-1 units/μL

wet ice

−70℃

100 u in Plastic ampoule

Recombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp³¹⁶, Asp³³⁰, and possibly Asp¹⁵². MW: 12000 and 19000.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-pNA (Cat. No. 218820) per h at 37°C, pH 7.2.

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.

Talanian, R.V., et al. 1997. J. Biol. Chem.272, 9677.
Wang, L., et al. 1994. Cell78, 739.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 2