Caspase-5, Human, Recombinant, E. coli

100

≥80% (SDS-PAGE)

liquid

≥100,000 units/mg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

3000 u in Plastic ampoule

Recombinant, human caspase-5 expressed in E. coli. A member of the caspase-1 subfamily of cysteine proteases involved in Fas-mediated apoptosis. Caspase-5 cleavage site is reported to be Asp³¹¹ and Asp³³⁰. May also cleave at Asp¹²¹. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.
M.W. 10,000 and 22,000 (heterodimer).
Recombinant, human caspase-5 expressed in E. coli. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-pNA (Cat. No. 218805) per min at 25°C, pH 7.4.

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

After initial defrost, aliquot and freeze at -70°C.

Schwartz, S. et al. 1999. Cancer Res. 59, 2995.
Fucheu, C., et al. 1996. Eur. J. Biochem. 236, 207.
Munday, N.A., et al. 1995. J. Biol. Chem. 270, 15870.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 2