β1-3,6-Galactosidase, Recombinant, E. coli

3.2.1.23 ( BRENDA | IUBMB )

Recombinant, β1-3,6-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,3- and β1,6-linked galactose from complex carbohydrates and glycoproteins. Not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Recombinant, β1-3,6-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,3-and β1,6-linked galactose from complex carbohydrates and glycoproteins. Not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

liquid

100

≥30 units/mL
≥75 units/mg protein

Calbiochem^®

do not freeze

Proteases, none detected

wet ice

2-8℃

2 u in Plastic ampoule

12 - Non Combustible Liquids

WGK 2