您的位置：首页 > 产品中心 > Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated
Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated
| 产品编号： | 4100012 |
| 规格： | clone TU-20, from mouse, ALEXA FLUOR® 488 |
| 包装规格： | 40 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated
Tubulin beta-4, Tubulin beta-III, tubulin, beta 3, tubulin, beta 4
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions. |
| Specificity【特异性】 | Reactivity with other species has not been determined. This antibody reacts with the C-terminus of the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells. |
| Immunogen【免疫原】 | Epitope: C-terminus |
| Application【应用】 | Detect Tubulin β-III using this Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated validated for use in IC, IF, WB, IH & IP. Immunohistochemistry: 5-10 µg/mL on frozen brain tissue. Although this conjugated antibody will work for Western blotting and Immunoprecipitation, we recommend catalog number CBL412 if these are your primary applications. Optimal working concentration must be determined by end user. Research Sub Category Neurofilament & Neuron Metabolism Research Category Neuroscience |
| Quality【质量】 | Routinely tested cortical neurons in Immunocytochemistry. Immunocytochemical Analysis: 1:250 dilution of this lot detected β-III tubulin in rat cortex cell culture. |
| Physical form【外形】 | Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.05% sodium azide. Protein G Purified |
| Analysis Note【分析说明】 | Control Rat Cortex Cells. |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Legal Information【法律信息】 | ALEXA FLUOR is a registered trademark of Life Technologies |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | ALEXA FLUOR® 488 |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | TU-20, monoclonal |
| species reactivity | bovine, primate, mouse, pig, human, rat |
| technique(s) | immunocytochemistry: suitable immunofluorescence: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG1 |
| NCBI accession no.【NCBI登记号】 | NP_001184110 |
| UniProt accession no.【UniProt登记号】 | Q13509 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | 50 kDa |
| Storage and Stability【储存及稳定性】 | Stable for 6 months at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
