Anti-Integrin α1 Antibody, clone FB12, azide free

32160702

NA.41

Reacts with the I domain (Val151 - Ala364) of human alpha1 integrin (Fabbri, 1996). Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi, 1991). Does not recognize recombinant I domain of rat integrin alpha1.

Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation. Immunoblotting: 2 μg/mL

Immunoprecipitation: 1 μg/mL

FACS analysis: 2 μg/mL

ELISA: 0.5 μg/mL

Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri, 1996): 1-5 μg/mL

Optimal working dilutions must be determined by end user.
Detect Integrin α1 using this Anti-Integrin α1 Antibody, clone FB12, azide free validated for use in ELISA, FC, IH, IP, FUNC & WB.

Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl.
Protein A purified
Format: Purified

Control
Microvascular endothelium

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

FB12, monoclonal

human

Chemicon^®

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1

NM_181501.1

P56199

wet ice

human ... ITGA1(3672)

130 kDa

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12 - Non Combustible Liquids

WGK 2