Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9

32160702

NA.41

Recognizes a protein of 135kDa, identified as the alpha-subunit of insulin receptor (IR). Its epitope localizes in exon 7/8 and shows no cross-reaction with IGF-receptors {Soos, 1986}. Antibody superbly inhibits (>90%) insulin binding and BLOCKS insulin action. IR is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a beta-a-a-beta configuration. The beta-subunit (95kDa) possesses a single transmembrane domain, whereas the alpha-subunit is completely extracellular. The intracellular portion of the beta-subunit is a tyrosine-specific protein kinase with sequence homology to other such kinases. Activation of kinase is essential in signaling pathway whereby insulin regulates intracellular metabolism.

IM-9 lymphocytes followed by purified insulin receptor.
Epitope: alpha subunit, azide free

Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9 detects level of Insulin Receptor & has been published & validated.
Completely (>90%) Inhibits Insulin Binding: use antibody at 10nM

Antagonist: Blocks insulin action

Does not work in immunoprecipitation {Soos, 1986}.

Optimal working dilutions must be determined by end user.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Protein G Purified mouse immunoglobulin in 10mM PBS, pH 7.4 contain no preservatives.
Format: Purified
Protein G Purified

Control
3T3 cells expressing transfected human insulin receptor

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

47-9, monoclonal

pig, sheep, human, bovine

mouse, rat

Chemicon^®

IgG1

not suitable for Western blot
not suitable for immunoprecipitation

NM_000208.2
NM_001079817.1

P06213

wet ice

human ... INSR(3643)

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2