Anti-Integrin β1 Antibody, activated, clone HUTS-4, Azide Free

32160702

NA.41

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Monoclonal antibody MAB2079Z is specific for active conformation of human beta1 integrins. The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit nonconvalently associated with the common beta1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies such as MAB2079Z which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. Antibody MAB2079Z recognizes epitopes in the 355-425 region of the common beta1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli.

Generated against human beta1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. MAB2079Z was specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.
Epitope: activated, azide free

Research Category
Cell Structure
Research Sub Category
Integrins
ELISA

Immunohistochemistry: fresh frozen, or light fixation, with 4% PFA permeabilized with 0.1% triton X-100 in the presence of Mn++ as the epitope of HUTS-4 is dependent upon Mn++ ion. Note antibody only stains activated beta1 integrins. Activated beta-1 integrins are typically found in wound regions for instance (see http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=eurekah.figgrp.12951) . Use MAB1981 or CBL1333 for general beta1 integrin staining and detection.

Western Blotting: non-reducing conditions only (see: http://www.jbc.org/cgi/content/full/271/19/11067).

FACS analysis: 2.5 μg antibody / 5 x 10⁵ cells.
Detect Integrin β1 using this Anti-Integrin β1 Antibody, activated, clone HUTS-4, Azide Free validated for use in ELISA, FC, IH and WB.

Format: Purified
Protein A Purified mouse immunoglobulin in PBS containing no preservatives.
Protein A purified

Control
Human lung, liver, and skeletal muscle tissues

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

HUTS-4, monoclonal

rat, human

Chemicon^®

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG2b

NM_002292.3

P05556

wet ice

88 kDa

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2