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Abl (M351T) Protein, active, 10 μg
| 产品编号： | 4101011 |
| 规格： | Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the M351T mutation. For use in Kinase Assays. |
| 包装规格： | 10 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.32 |
| General description【一般描述】 | Product Source: N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the M351T mutation. Expressed by baculovirus in Sf21 insect cells. The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the M351T mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125). |
| Biochem/physiol Actions【生化/生理作用】 | Protein Target: Abl Target Sub-Family: TK |
| Quality【质量】 | Routinely evaluated by phosphorylation of Abltide. |
| Physical form【外形】 | Ni2+/NTA-agarose 50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution. |
| Other Notes【其他说明】 | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in baculovirus infected Sf21 cells |
| mol wt【分子量】 | Mw 121 kDa |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable (kinase) |
| UniProt accession no.【UniProt登记号】 | P00519 U07563 |
| shipped in【运输】 | dry ice |
| packaging【包装】 | Also available in 250μg size as catalog # 14-757M - Call for pricing and availability. |
产品说明
| Storage and Stability【储存及稳定性】 | Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap. Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Pictograms【象形图】 | GHS07 |
| Signal word【警示用语：】 | Warning |
| Hazard Statements | H317 |
| Precautionary Statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| Hazard Classifications【危险分类】 | Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
