Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor^® 488 conjugate

32160702

NA.41

Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.

Recognizes α-Tubulin, clone DM1A, Mr 50-60 kDa.

Epitope: The epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).

Western Blot Analysis:
2 µg/mL of a previous lot detected α-Tubulin in RIPA lysates from A431 cells.

Immunocytochemistry:
2 µg/mL of a previous lot showed positive immunostaining for α-Tubulin in HeLa cells.
Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.

Routinely evaluated by Flow Cytometry.

Flow Cytometry:
0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.

Alexa Fluor^® 488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween-20, 0.05% sodium azide.
Liquid at 2-8°C.
Protein G Purified

Control
Included Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor^® 488-conjugate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

ALEXA FLUOR is a registered trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

ALEXA FLUOR^® 488

purified antibody

primary antibodies

DM1A, monoclonal

mouse, guinea pig, pig, human, rat, avian, bovine

Upstate^®

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

IgG1

NP_006000

Q71U36

wet ice

human ... TUBA1A(7846)

50-60 kDa

Stable for 1 year at 2-8°C from date of receipt.
Do Not Freeze. Do not store the material diluted.
For maximum recovery of product, centrifuge original vial prior to removing cap.

Alexa Fluor^® is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2