Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate

32160702

NA.41

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Recognizes Akt1/PKBα.

GST-fusion protein corresponding to residues 1-149 of human Akt1

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Evaluated by immunoprecipitation on 250 μg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).

Protein G Chromatography
20% slurry; 1X PBS, 0.05% sodium azide.
Sufficient for 10 IPs.
Format: Purified

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

100

mouse

purified antibody

primary antibodies

SKB1, monoclonal

human, mouse, rat

immunoprecipitation (IP): suitable

IgG

wet ice

~ 60 kDa observed

Stable for 1 year at 2-8ºC from date of receipt.

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10 - Combustible liquids

WGK 2