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Rabbit IgG Isotype Control ColorWheel® Dye-Ready pAb
产品编号: | 4101739 |
规格: | for use with ColorWheel® Dyes (Required, sold separately) |
包装规格: | 25 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | N/A, polyclonal |
product line【产品线】 | ColorWheel® |
form【形式】 | lyophilized |
packaging【包装】 | antibody small pack of 25 μL |
technique(s) | flow cytometry: suitable |
isotype【同位素/亚型】 | IgG |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | 2-8℃ |
compatibility【相容性】 | Requires ColorWheel® Dye (Sold Separately) |
基本信息
General description【一般描述】 | ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application. |
Specificity【特异性】 | This rabbit polyclonal antibody can be used as an isotype control for Rabbit IgG. |
Application【应用】 | Quality Control Testing: Evaluated by Flow Cytometry with Protein A/G beads. Rabbit IgG Isotype Control ColorWheel® Dye-Ready pAb was tested by flow cytometry using Protein A/G beads. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
Physical form【外形】 | Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide. |
Reconstitution【重悬】 | 1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
Legal Information【法律信息】 | ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品说明
Target description【目标描述】 | Isotype control antibodies are ideal negative controls for various immunological applications. They can help discriminate between specific antibody signal from non-specific background signal. In selecting an isotype control consideration of host, isotype, conjugation, light chain, and concentration are important. It should be of the same species and isotype as the antibody of interest and must be used at the same concentration and conjugated to the same fluorophore. Rabbit IgG Isotype Control is suitable for use an isotype-matched control for Rabbit IgG and can be used for evaluation of background staining caused by non-specific interactions between a rabbit IgG antibody and the sample under defined experimental conditions. |
Storage and Stability【储存及稳定性】 | Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 3 |