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Rac1 Activation Assay Kit
产品编号: | 4101745 |
规格: | Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody. |
包装规格: | 1 KIT |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
eCl@ss | 32161000 |
NACRES | NA.32 |
General description【一般描述】 | Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Ras-related C3 botulinum toxin substrate 1 (Rac1)-GTP from cell lysates and detected by a Rac1 specific monoclonal antibody. Rac1 belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). It is expressed in two major forms such as an inactive GDP-bound form and an active GTP-bound form. Rac1 is involved in influencing several physiological conditions by interacting with effectors that activate several signaling cascades. The RAC1 gene is located on the human chromosome at 7p22.1. |
Application【应用】 | Rac1 Activation Assay Kit has been used in the detection of active Ras-related C3 botulinum toxin substrate 1 (Rac1) in brain homogenates of mice and humans by immunoblotting. |
Components【组分】 | Anti-Rac1, clone 23A8 (Cat.# 05-389) Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168) 100X GTPγS, 10mM (Cat.# 20-176) 100X GDP, 100mM (Cat.# 20-177) Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325) |
Quality【质量】 | Routinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac. |
Linkage【联系】 | Replaces: SGT425 |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
species reactivity | rat, human, mouse |
manufacturer/tradename | Upstate® |
technique(s) | activity assay: suitable affinity binding assay: suitable |
NCBI accession no.【NCBI登记号】 | NM_006908.3 NM_018890.2 NM_198829.1 |
UniProt accession no.【UniProt登记号】 | P63000 |
shipped in【运输】 | dry ice |
Gene Information | human ... RAC1(5879) |
产品说明
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |