Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5

32160702

NA.41

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Expected to cross-react with porcine, chicken, bovine, and rabbit.
The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).

Purified glial filament (Debus, E., 1983).

Research Sub Category
Neuronal & Glial Markers
Immunocytochemistry:
5 μg/mL of a previous lot was used.

Immunohistochemistry:
5 µg/mL

Immunoblotting:
Recognizes a 51kDa protein in reducing westerns of total brain lysates.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB with more than 55 product citations.

Routinely evaluated by Western Blot on Mouse brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 μg of Mouse Brain lysate.

Replaces: 04-1031; 04-1062

Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
Format: Purified
Protein A purified

Control
Mouse brain tissue, Astrocyte culture.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

GA5, monoclonal

chicken, rat, rabbit, mouse, pig, bovine, human

Chemicon^®

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG1

NM_002055.2

P14136

wet ice

human ... GFAP(2670)

50 kDa

Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
DO NOT FREEZE.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2