广州伟伯科技有限公司
您的位置:首页 > 产品中心 > Anti-APP Antibody, APP 643-695 CT fragment, clone 2.F2.19B4
产品搜索:

Anti-APP Antibody, APP 643-695 CT fragment, clone 2.F2.19B4

产品编号:4102166
规格:ascites fluid, clone 2.F2.19B4, Chemicon®
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4102166100 μL7190
产品别名

Anti-APP Antibody, APP 643-695 CT fragment, clone 2.F2.19B4

APP, Jonas clone

基本信息
eCl@ss
32160702
NACRES
NA.41
Specificity【特异性】
Reacts with intact full-length Alzheimer precursor protein (APP) and selectively with the cytoplasmic carboxyl fragment of APP 643-695. Epitope has reportedly been mapped in this paper http://www.impub.co.uk/abs/W386.html
Immunogen【免疫原】
Epitope: APP 643-695 C-terminal fragment
Carboxyl fragment of APP 643-695 / Jonas.
Application【应用】
Research Category
Neuroscience
This Anti-APP Antibody, APP 643-695 C-terminal fragment, clone 2.F2.19B4 is validated for use in IH(P), WB for the detection of Alzheimer Precursor Protein.
Immunohistochemistry on paraffin sections: 10-20 μg/mL * See protocol below.

Western blot: 10 μg/mL

Optimal working dilutions must be determined by end user.

APPLICATION NOTES FOR MAB343

IMMUNOHISTOCHEMISTRY

1) Prepare paraformaldehyde-fixed paraffin sections. Wash twice for 5 min in xylene to deparaffinize. Wash sections for 5 min in a descending series of alcohol solutions (100%, 96%, 90%, 80%, 70%, 50%, 30%).

2) Wash sections 3 times in distilled water.

3) Wash in TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.6). To block endogenous peroxidase wash with methanol containing 0.6% H2O2 (v/v) and 10 % horse serum (v/v) for 5 min at room temperature.

4) Wash sections for 5 min in TBS.

5) Incubate sections with MAB343 (diluted in TBS containing 10% horse serum (v/v)) overnight at +4°C or for 2 hours at 37°C in a humid chamber.

6) Wash sections 3 times in TBS for 5 min..

7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).

8) Wash sections in TBS.

9) Embed sections and examine.
Research Sub Category
Neurodegenerative Diseases
Physical form【外形】
Unpurified
Liquid.
Analysis Note【分析说明】
Control
Brain
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
ascites fluid
clone【克隆】
2.F2.19B4, monoclonal
species reactivity
human, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype【同位素/亚型】
IgG1
NCBI accession no.【NCBI登记号】
NM_000484.2
NM_201413.1
NM_201414.1
UniProt accession no.【UniProt登记号】
P05067
shipped in【运输】
dry ice
Gene Information
human ... APP(351)
产品说明
Storage and Stability【储存及稳定性】
Maintain lyophilized material at +2–8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.0079