Anti-Collagen Type I Antibody

32160702

NA.41

Recognizes Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.

Dot and slot blotting: 1:100-1:500

ELISA: 1:50-1:100

Indirect immunohistochemistry (frozen sections only): 1:10-1:20

Indirect immunocytochemistry: 1:10-1:20

Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.
Anti-Collagen Type I Antibody is an antibody against Collagen Type I for use in DB, ELISA, IC, IH & WB.

ImmunoAffinity Purified
Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose^® 4B.. Liquid in 0.5 mL of 100 mM borate buffered saline, pH 8.0, no preservatives.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a registered trademark of Cytiva

100

goat

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

bovine, human

Chemicon^®

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

not suitable for immunohistochemistry (Paraffin)

NP_000079

P02452

wet ice

human ... COL1A1(1277)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

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