Anti-Collagen Type I Antibody, biotin conjugated

32160702

Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758B has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Human placental collagen type I

Research Category
Cell Structure
Dot and slot blotting: 1:500-1:1,000

ELISA: 1:1,000-4,000

Indirect immunohistochemistry (frozen sections only): 1:20-1:40

The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.
Research Sub Category
ECM Proteins
Anti-Collagen Type I Antibody, biotin conjugated is an antibody against Collagen Type Id for use in DB, ELISA, WB, IH.

Affinity purified immunoglobulin, biotin labeled. Prior to purification the antisera was adsorbed against collagen type II, III, IV, V and VI immobilized on Sepharose^® 4B.. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% sodium azide.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a registered trademark of Cytiva

100

goat

biotin conjugate

affinity purified immunoglobulin

primary antibodies

polyclonal

human, bovine

Chemicon^®

ELISA: suitable
dot blot: suitable
immunohistochemistry: suitable
western blot: suitable

not suitable for immunohistochemistry (Paraffin)

NM_000088.3

P02452

wet ice

human ... COL1A1(1277)

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2