Anti-DNA Antibody, double stranded, clone BV16-13

32160702

NA.41

Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.

BINDING ASSAY:

Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.



ANTIGEN BINDING

PROFILE: Relative Binding To:



Natural DNA Synthetic homopolymers

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01



Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).



a) Native calf thymus DNA b) Heat-denatured calf thymus DNA

Epitope: double stranded, 28% reactive with single stranded

EIA/RIA

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair

Apoptosis - Additional

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

culture supernatant

primary antibodies

BV16-13, monoclonal

all

Chemicon^®

ELISA: suitable
immunocytochemistry: suitable
radioimmunoassay: suitable

IgG2aκ

wet ice

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1