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Anti-E-Cadherin Antibody, clone 67A4

产品编号:4102322
规格:clone 67A4, Chemicon®, from mouse
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4102322100 μG5420
产品别名

Anti-E-Cadherin Antibody, clone 67A4

CD324

基本信息
eCl@ss
32160702
NACRES
NA.41
Specificity【特异性】
Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E-cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.

Antigen is expressed in non-neural epithelial tissues.
Immunogen【免疫原】
T-47D breast carcinoma line
Application【应用】
Research Category
Cell Structure
Anti-E-Cadherin Antibody, clone 67A4 detects level of E-Cadherin & has been published & validated for use in FC & WB.
Immunoblotting: 123kD in membrane fractions; MCF-7 membranes positive control. Confluent cells were washed three times with ice-cold PBS and lysed in 50mM HEPES pH 7.5, 150mM NaCl, 1mM EDTA, 10% glycerine, 1% triton X-100, 20mM Na(4)P(2)O(7), 2mM Na-vanadate, 100mM NaF, 2mM PMSF, 20μg/mL leupeptin, 1mM pNPP(para-nitrophenylphosphate), 20μg/mL aprotinin, and 200μM NH(4)-molybdate. Crude extracts were clarified by centrifugation (10,000 x g 10 min), and supernatants were taken for immunoprecipitation. Protein concentration of cleared lysates were determined by BCA protein assay. {Buhring, et al Luekemia 10:106-116, 1996}.



FACS Analysis

Functional blocking of E-cadherin

Does not work well for immunoprecipitation

Original paper, Buhring, H et al Leukemia 10:106-116, 1996.

Optimal working dilutions must be determined by end user.
Research Sub Category
Adhesion (CAMs)
Linkage【联系】
Replaces: 04-1103
Physical form【外形】
Protein A purified immunoglobulin. Liquid in 0.02M PBS with NaCl, pH=7.6, 0.1% Sodium azide.
Format: Purified
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
67A4, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
western blot: suitable
isotype【同位素/亚型】
IgG1
NCBI accession no.【NCBI登记号】
NM_004360.2
UniProt accession no.【UniProt登记号】
P12830
shipped in【运输】
wet ice
产品说明
Storage and Stability【储存及稳定性】
Maintain 2-8°C in undiluted aliquots for up to 12 months after date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
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