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Anti-GAL4 Antibody (activation domain)
| 产品编号： | 4102383 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-GAL4 Antibody (activation domain)
Regulatory protein GAL4
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | GAL4 is a transcriptional activator which controls the expression of genes involved in the catabolism of galactose. In addition to a DNA-binding domain, Gal4 contains two acidic regions: activating region I (aa148-238) and activating region II (aa768-881), which can both activate transcription when independently joined to the DNA-binding domain. The activation domains of Gal4 have been shown to interact with Gal80, TBP, SUG1, and ADA2, among other general transcription factors. |
| Specificity【特异性】 | This antibody recognizes the C-terminus of GAL4 (activation domain). |
| Immunogen【免疫原】 | GST-tagged recombinant protein corresponding to the C-terminus of yeast GAL4 (activation domain). Epitope: C-terminus |
| Application【应用】 | Research Sub Category Cell Cycle, DNA Replication & Repair Research Category Epigenetics & Nuclear Function Use Anti-GAL4 (activation domain) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect GAL4 (activation domain) also known as Regulatory protein GAL4. |
| Quality【质量】 | Evaluated by Western Blot in Gal4 positive and Gal4 negative yeast cell lysate. Western Blot Analysis: A 1:160,000 dilution of this antibody detected Gal4 in 5 mg of Gal4 positive (+ recombinant Gal4) yeast cell lysate. This antibody also detected small mounts of endogenous Gal4 in Gal4 negative (- recombinant Gal4) yeast cell lysate. |
| Linkage【联系】 | Replaces: 06-283 |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control Gal4 positive and Gal4 negative yeast |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | yeast |
| technique(s) | western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_015076 |
| UniProt accession no.【UniProt登记号】 | P04386 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~28 kDa, ~38 kDa, ~77 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
