Anti-GLAST Antibody, clone 8C11.1

32160702

Excitatory amino acid transporter 1 (UniProt P43003; also known as GLAST-1, Sodium-dependent glutamate/aspartate transporter 1, Solute carrier family 1 member 3) is encoded by the SLC1A3 (also known as EA6, EAAT1, GLAST, GLAST1) gene in human (Entrez Gene ID 6507). GLAST-1 is a multitransmembrane protein that functions as a high affinity glutamate transporter and mediates the termination of postsynaptic action of glutamate in central nervous system by rapidly removing released glutamate from the synaptic cleft. GLAST-1 also transports L- and D-aspartate. GLAST gene mutations are linked to episodic ataxia type 6 (EA6), a disorder characterized by seizures, migraine, and alternating hemiplegia. There are 5 known spliced variants. Isoform 2 has no functional glutamate uptake activity and exerts a dominant negative effect against isoform 1.

Clone 8C11.1 epitope lies within the second extracellular segement of GLAST isoforms 1 & 2. The epitope region is also present in isoform 4, but not in isforms 3 & 5.

GST-tagged recombinant protein corresponding to the extracellular domain of human GLAST.
Epitope: Extracellular domain

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLAST in human cerebellum and rat cerebellum tissue.
Anti-GLAST Antibody, clone 8C11.1 is an antibody against GLAST for use in Western Blotting, Immunohistochemistry .
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GLAST in 200 µg of human brain tissue lysate.

Format: Purified
Purified mouse monoclonal IgG1λ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified antibody

primary antibodies

8C11.1, monoclonal

human, rat

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG1λ

NP_004163

P43003

wet ice

human ... SLC1A3(6507)

~55 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1